段艳凤,文国宏,Armstrong M R,李广存,金黎平

• 1:中国农业科学院蔬菜花卉研究所
• 2:甘肃省农业科学院马铃薯研究所
• 3:英国邓迪大学/詹姆斯赫顿研究所植物科学系

摘要(Abstract)：

为挖掘抗晚疫病马铃薯资源,研究利用4个不同毒力的晚疫病菌株,基于离体叶片接种法对四倍体材料‘陇薯12号’和‘L05Nsr-j-1’进行抗性鉴定。结果表明‘陇薯12号’高抗除"超级生理小种"CN152外的其余3个菌株,‘L05Nsr-j-1’则对4个菌株均表现为高感;进一步利用晚疫病菌效应子E38在上述2份材料上进行农杆菌介导的瞬时表达,发现只有‘陇薯12号’可以识别E38产生特异的HR反应;抗病基因富集测序诊断(dRenSeq)结果表明‘陇薯12号’不含已知的晚疫病抗病基因,预示‘陇薯12号’含有未知的抗病基因;利用E38对‘陇薯12号’和‘L05Nsr-j-1’的F1群体215个单株进行农杆菌介导的瞬时表达检测,结果显示67%的单株出现假阳性反应,表明其F1群体不适合利用农杆菌介导的效应子识别鉴别基因型。研究结果为今后‘陇薯12号’的利用及其晚疫病抗性基因的克隆奠定了基础。

关键词(KeyWords)： 马铃薯;晚疫病;效应子;抗病基因

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系专项资金资助(CARS-9)

作者(Author): 段艳凤,文国宏,Armstrong M R,李广存,金黎平

DOI: 10.19918/j.cnki.1672-3635.2021.02.001

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