马铃薯抗青枯病体细胞变异体离体筛选研究初报A PRELIMINARY STUDY ON SCREENING POTATO SOMATIC VARIANT IN VITRO FOR RESISTANCE TO BACTERIAL WILT
何礼远
摘要(Abstract)：
本试验证明了青枯菌活菌作为马铃薯细胞变异体离体筛选的选择压力是适宜的。明确了活菌压力的最适浓度、愈伤组织接种的最适龄期和接种的方法。愈伤组织培养皿内继代培养重复接种筛选是快速而有效的筛选方法。从近2万块马铃薯叶圆片愈伤组织中,经重复接种筛选获得了6块抗菌愈伤组织(比率为0.03％左右)。并经过分化诱导培养获得了再生苗。初步的抗性鉴定结果表明,再生苗抗1(PSS—1)比母体(米拉脱毒试管苗)的抗病性有显著提高。
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作者(Author): 何礼远
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