王明,王万兴,何长征,胡新喜,熊兴耀,秦玉芝

• 1:湖南农业大学园艺园林学院
• 2:中国农业科学院蔬菜花卉研究所

摘要(Abstract)：

依据马铃薯转录组数据,鉴定马铃薯脯氨酸转运体(Proline transporter,ProTs)在逆境中的作用。以马铃薯GS393(Solanum commersonii-LZ3.4-Wisconsin, United States)为材料,采用实时荧光定量PCR对StProT3基因在不同组织及激素、重金属、盐、干旱和低温处理下的表达模式进行分析。该基因CDS长度为1 317 bp,编码438个氨基酸。序列比对显示与SlyProT3有96%的相似性,因而命名为StProT3(NCBI登陆号为MH027990.1)。StProT3基因在马铃薯根、茎、叶、匍匐茎以及块茎中均有表达,其中根中表达量最高;重金属(氯化汞、氯化镉、氯化铜、氯化铝)、干旱、盐渍、激素(IAA、GA3、6-BA)处理能诱导该基因表达,而低温和ABA处理则抑制该基因表达,这表明StProT3基因参与了马铃薯的激素信号传导以及非生物胁迫响应。

关键词(KeyWords)： 马铃薯;脯氨酸转运体;实时荧光定量;非生物胁迫

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31371683)

作者(Author): 王明,王万兴,何长征,胡新喜,熊兴耀,秦玉芝

DOI: 10.19918/j.cnki.1672-3635.2021.02.003

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