用根癌土壤杆菌和发根土壤杆菌转化马铃薯的初步研究POTATO GENETIC TRANSFORMATION USING AGROBACTERIUM TUMEFACIENS AND A. RHIZOGENES
王春林,朱明凯,程天庆
摘要(Abstract)：
根癌土杆菌菌株T_(37),C_(58)和B_6S_3单独或与C_(58) CIpGV 3850::110(?)neo(带有一嵌合基因NOS—NPT使植物产生卡那霉素抗性)接种马铃薯块茎盘。每块茎盘肿瘤数因细菌菌株、马铃薯基因型和所取块茎盘在块茎上的位置而变化。T_(37)诱导的品种Desiree的肿瘤分化了芽。大部分肿瘤的分化芽合成胭脂碱,表明是经pTiT—DNA转化的。用发根土壤杆菌菌株A_4诱导的毛状根切段培养后再生成株。再生植株具有明显的表型变异,并有agropinc合成,证明是经pRiT—DNA转化的。
关键词(KeyWords)：
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作者(Author): 王春林,朱明凯,程天庆
参考文献(References)：
- 1 Caplan A et al. Science, 1°83, 222: 815～821
- 2 Nester E W and Kosuge T. Ann Rev Microbiol, 1981, 35:531～565
- 3 Bevan WB and Chilton MD. Ann Rev Genet, 1982, 367～384
- 4 Binns A N. Oxford Surveys of Plant Moleculan and Cell Biology, 1984, 1:133～160
- 5 Cheysen G et al. Genetic Flux in Plants, Spring Verlaig, Wien, PP. Hohn B, Dennis ES [eds] Advances in Plant Gene Research, 1985, 2:11～47
- 6 Zambryski P et al. Mobile Genetic Elements New York: Academic Press, 1983
- 7 Ham et al. MGG, 1985, 199: 161-168
- 8 Birot AM et al. Plant Physiol Biochem, 1987, 4 : 853～859
- 9 Tepfer M and Casse-delbart F. Microbiol Sci, 1987, 4: 24～28
- 10 Akiyoshi D et al. PNAS, 1984, 81:5994～5998
- 11 Buchmann I et al. EMBO J, 1985, 4 : 853～859
- 12 Constantino P et al. J Mol Appl Genet, 1984 2(1) : 43～51
- 13 David C et al. Biotech, 1984, 2 : 73～76
- 14 Camai L et al. Nature, 1985, 37 : 741～744
- 15 Morgan AJ et al. Plant Sci, 1987, 49 : 37～49
- 16 Simpson RB et al. Plant Mol Blol, 1986, 6 : 403～415
- 17 Sacristam MD and Melchers G. MGG, 1987, 152 : 111～117
- 18 Jaynes JM et al. TIBECH, 1986, 314～320
- 19 Sheerman S and Bevan M W. Plant Cell Rep, 1988, 7: 13～16
- 20 Stiekema W J et al. Plant cell Rep, 1988, 7: 47～50
- 21 Dai C X and Pueppke S P. CMICN, 1986, 2(1) : 43～45
- 22 White DWR and Greenwood D. Plant Mol Biol, 1987, 8 : 461～468
- 23 Ooms G et al. Cell, 1983, 30: 589～597
- 21 Schafer W et al. Nature, 1987, 327: 529～532
- 25 Van Slogteren G M S et al. Plant Mol Biol, 1983, 2 : 321～335
- 26 De Greve H et al. Nature, 1982． 300 : 752～755
- 27 Uhrig H and Salamini I. Z Pflanzenzuch,1987, 91: 211～218
- 28 Wenzel G and Uhrig H. TAC, 1981, 59: 333～340
- 29 Ooms G et al. Potato Res, 1936, 29: 367～379
- 30 David C and Tempe J. Plant Cell Rep, 1988． 7: 88～91
- 31 Hanischten Cate C H et al. Plant Sci, 1987, 49: 217～222
- 32 Hanischten Cate CH and Ramalu K S. Plant Sci, 1987, 49: 209～216
- 33 Ooms G and Lenton J R. PMB Plant Mol Biul, 1985, 5: 205～212
- 34 Ooms G. TAG, 1987, 73: 744～750
- 35 Horsch RB et al. Science, 1985, 227: 1229～1231
- 36 Shahin E A et al. Hort Sci, 1986, 21:1199～1201