马铃薯腺苷二磷酸葡萄糖焦磷酸化酶研究进展
成善汉,柳俊,谢从华
摘要(Abstract):
腺苷二磷酸葡萄糖焦磷酸化酶 (AGPase)是马铃薯淀粉合成的限速酶 ,有关该酶的酶学特性、表达规律及分子生物学已进行了较为深入和系统的研究。异源表达和突变研究表明 ,该酶的小亚基具催化活性 ,大亚基具变构调节作用。化学修饰和位点定向突变方法分析已发现了AGPase的底物、激活因子和抑制因子结合位点。此外 ,通量控制系数、反义抑制和反向遗传学等多种方法已阐述了该酶是淀粉合成关键酶的原因
关键词(KeyWords): 马铃薯;腺苷二磷酸葡萄糖焦磷酸化酶;亚基;结合位点;淀粉合成
基金项目(Foundation):
作者(Author): 成善汉,柳俊,谢从华
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