张威,魏旭言,高艳玲,范国权,邱彩玲,孙旭红,武新娟,张俐俐,白艳菊

• 1:黑龙江省农业科学院
• 2:昭通学院农学与生命科学学院

摘要(Abstract)：

马铃薯A病毒(Potato virus A,PVA)是马铃薯生产上较常见的病毒之一,植株感病症状与品种、病毒株系和环境条件有关。为研究PVA不同分离物对马铃薯的致病力,将3种遗传背景不同的PVA分离物纯毒源,分别接种到指示植物‘黄花烟’和马铃薯品种‘克新13号’上,应用qRT-PCR检测技术进行病毒侵染鉴定,并观察其致病性差异。结果表明,3种PVA分离物均可成功侵染‘黄花烟’和‘克新13号’,通过接种后‘黄花烟’叶片的症状表现,推断3种分离物的致病性具有一定差异。在马铃薯上分离物2017-171致病力表现为最强,不仅在植株体内病毒含量最高,可致使叶片出现叶缘皱缩、轻花叶,还能导致马铃薯块茎严重裂口、芽眼变深;其次是分离物KB117,病毒侵染速度较快但病毒含量低,植株症状相对明显,叶缘变尖、波浪状皱缩,块茎发生畸形且芽眼变深;最后是分离物2017-176,植株仅个别叶片呈现轻微叶缘皱缩,块茎未出现畸形只存在芽眼变深症状,病毒侵染速度相对慢但病毒含量居中。PVA不同株系的致病性分析为其有效防治提供了有价值的理论依据。

关键词(KeyWords)： 马铃薯A病毒(PVA);分离物;病毒含量;感病症状;致病力

Abstract:

Keywords:

基金项目(Foundation): 财政部和农业农村部：国家现代农业产业技术体系资助（CARS-09）;; “农业科技创新跨越工程”专项（HNK2019CX07）

作者(Author): 张威,魏旭言,高艳玲,范国权,邱彩玲,孙旭红,武新娟,张俐俐,白艳菊

DOI: 10.19918/j.cnki.1672-3635.2021.06.012

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