刘圣宣,程云霞,刘腾飞,宋波涛

• 1:农业农村部马铃薯生物学与生物技术重点实验室/湖北马铃暮工程技术研究中心/华中农业大学
• 2:塔里木大学植物科学学院

摘要(Abstract)：

马铃薯通常被认为是干旱敏感性作物,由全球气候变化引起的干旱频发严重威胁了马铃薯的可持续生产。破译马铃薯的干旱应答机制,解析马铃薯干旱应答的核心因子,将有助于破解因干旱导致的马铃薯种植限制。基于此,研究旨在通过分析栽培品种应对干旱胁迫的早期响应过程,获得马铃薯早期干旱应答的关键候选基因。将‘鄂马铃薯3号’试管苗通过快速干旱胁迫处理,可在6 h出现明显的干旱胁迫表型,含水量相较于对照前也显著降低。不同干旱处理时间点的转录组分析结果显示,差异基因数量随着处理时间的延长而增加。2个分别编码转录因子GATA和YABBY的基因在干旱处理6 h后表达量极显著降低,可能为马铃薯干旱调控的负向因子。3个胚胎发育晚期富集蛋白(Late embryogenesis abundant,LEA)基因的表达受到干旱胁迫的剧烈诱导可作为潜在的干旱胁迫Marker基因。脱落酸(Abscisic acid,ABA)信号途径中的蛋白激酶StSnRK2.4和StSnRK2.8以及转录因子StAREB2受到干旱诱导最为剧烈,可能是ABA信号途径上响应干旱胁迫过程中的重要组分。

关键词(KeyWords)： 马铃薯;干旱胁迫;转录组分析;转录因子;ABA信号途径

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年科学基金项目（32101781）;; 国家农业（马铃薯）产业技术体系岗位科学家项目（CARS09-P07）;; 湖北省第三批现代农业产业技术体系项目（HBHZD-ZB-2020-005）

作者(Author): 刘圣宣,程云霞,刘腾飞,宋波涛

DOI: 10.19918/j.cnki.1672-3635.2021.06.001

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